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Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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GemPharmatech Co Ltd il-17b gene knockout (il-17b-/-) mice
Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Biotechnology Information protein sequences of il-17b
Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Labeo Technologies Inc il-17b protein
Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Novus Biologicals anti il17b
Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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R&D Systems rhil 17b
Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of <t>IL-17B,</t> TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.
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Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of IL-17B, TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.

Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of IL-17B, TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.

Article Snippet: The membrane was blocked with 5% skim milk prepared in Tris-buffered saline with Tween (TBST), followed by overnight incubation with primary antibodies against IL-17B (1:800, BOSTER), NF-κB (1:2000, Proteintech), TNF-α (1:800, BOSTER), and IL-1β (1:1000, Proteintech).

Techniques: Sequencing, Biomarker Discovery, Expressing